Kalki Subramaniam has been the champion of transgender rights in India. She has been working for the transgender community's equal rights and social justice since 2005. In the past ten years, she has spoken to more than a million students in Universities and colleges in India sensitizing them on gender diversity and seeking acceptance for gender queer people. She has made numerous documentations on LGBT rights. Her short documentary 'Her name is Sowmya' is a touching portrayal of the tragic death of her best friend transwoman Sowmwya. It was screened at several venues including the Bangalore Queer Film Festival'.

In 2010, Kalki was offically invited by the government of the United States of America under the International Visitors Leadership Program and traveled to Washington D.C, New York and SaltLake city. During her visit, she had interactions at Amnesty International, Human Rights Watch and New York Commission of Human Rights etc.

Kalki's milestone victory has been the Supreme court's verdict recongising transgender people legally. Kalki was one of the few activists who campaigned fiercely among the Judiciary of the country for the legal recognition of transgender people. On April 15th 2014, the Supreme Court of India legaly recognised transgender people. Kalki has worked along with the Ministry of Social Welfare of Tamilnadu, Kerala and Chatisgarh in recommending welfare measures and policies for transgender people.

In 2012, Kalki received an official invitation from the President of India to take part in the swearing in ceremony of the then Chief Justice of India, Shri. Altamas Kabir at Rashtrapati Bhavan, New Delhi.

On March 8th 2015, Facebook chose her as one of the 12 inspirational women of the world who have been using Facebook for community development. The Logical Indian website chose Kalki as the calander girl for the 12Humans.org initiative and said Kalki 'is the pride of the nation'. 

On February 2016, Kalki was nominated by NDTV and L'Oreal Paris for the Women Of Worth awards under the Arts category.